Metabolism of [14C]glucose by postimplantation mouse embryos in vitro.
نویسندگان
چکیده
Carbon dioxide and lactate production from [14C]glucose were measured for post-implantation mouse embryos aged 6 to 9 1/2 days post coitum in static cultures with a defined medium. The rate of metabolism increased rapidly and paralleled the increase in protein content indicating a fairly uniform rate of metabolism throughout the period. At all stages studied more than 90% of the glucose utilized was converted to lactate. Over a quarter of carbon dioxide produced was derived from the C-1 position resulting in high C-1:C-6 ratios, indicating that the Pentose Phosphate Shunt is a major oxidative pathway. The influence of various culture condition on CO2 production showed that high concentrations of glucose did not affect glucose utilization whilst high lactate concentrations had a significant inhibitory effect. Pyruvate had no discernible effect.
منابع مشابه
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متن کاملMetabolism of [C]glucose by postimplantation mouse embryos in vitro
Carbon dioxide and lactate production from [C]glucose were measured for postimplantation mouse embryos aged 6 to 9| days post coitum in static cultures with a defined medium. The rate of metabolism increased rapidly and paralleled the increase in protein content indicating a fairly uniform rate of metabolism throughout the period. At all stages studied more than 90 % of the glucose utilized was...
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ورودعنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 74 شماره
صفحات -
تاریخ انتشار 1983